Analytical Transparency
Each PRG compound is tested for purity and identity under EU analytical standards.
COA documentation — Coming soon — and can be provided upon request with your order.
Skip to product information
O-304 (ATX-304, OS-01) - High Purity Research Molecule (150 MG)
€145,00
Shipping calculated at checkout.
Due to its unique in vitro and in vivo regulation of AMPK, it is positioned not only for metabolic improvement but also for cardiovascular protection, exercise physiology, and aging research. It is considered a potential.
O-304 is a first-in-class, orally available pan- AMPK activator, which
increases AMPK activity by suppressing the dephosphorylation of pAMPK. O-304 exhibits a great potential as an agent to treat type 2 diabetes (T2D) and associated cardiovascular complications. Studies have found that aged mice fed it showed significantly improved cardiac function indicators, such as cardiac output, ejection fraction, and infarct volume, compared to the control group. This improvement was also accompanied by a reduction in insulin resistance and hyperinsulinemia, reflecting its potential to promote cardiac metabolism, energy utilization, and overall metabolic homeostasis. AMPK Activator O-304 protects against kidney aging through promoting energy metabolism and autophagy. O-304 reduces whole-body fat mass and lowers blood cholesterol levels in models of nonalcoholic steatohepatitis.
O-304 increases glucose uptake in skeletal muscle, reduces β-cell stress, and
promotes β-cell rest in diet-induced obese mice. O-304 reduces fasting plasma glucose levels. In a Phase IIa clinical trial in patients with T2D, it not only improved glucose homeostasis but also significantly enhanced peripheral microcirculatory function, including skin microvascular perfusion, which is a common mechanism leading to the severe consequences of T2D.
The small molecule pan-AMPK activator prevents dephosphorylation of AMPK at threonine-172. Muscle glucose uptake increases without needing insulin. Fat oxidation ramps up while fat synthesis is suppressed. O-304 has also been observed to promote autophagy and alleviate mitochondrial dysfunction. For example, in a model of oxidative stress, it significantly increased the formation of LC3B-positive autophagosomes, promoting autophagic flux, and restoring the expression of key factors involved in mitochondrial biogenesis (such as PGC-1α and TFAM), reducing
ROS production, and restoring mitochondrial ultrastructure, suggesting a protective role in maintaining cellular quality control and energy metabolism. Improves blood flow via nitric oxide signaling. It also acts as a mild mitochondrial uncoupler, increasing basal oxygen consumption and calorie burn by ~38% in cell models. O-304 also does not reduce cellular ATP levels, distinguishing it from indirect AMPK activators (metformin) that alter energy status. This behavior mimics the protective effect of ADP, unlike classic AMPK activators, which rely on reduced ATP or increased AMP levels for activation. (PP2C dephosphorylation experiments have shown that it can stabilize p-T172 AMPK even in the presence of high ATP levels.). Notably, this mechanism renders it a more gentle and sustained AMPK activation
tool, thereby preventing cellular errors or damage caused by energy depletion.
HED (Human Equivalent Dose):
Capsules are typically standardized to 100mg each, making the protocol simple:
Take 1–3 capsules daily(100–300mg). Best taken first thing in the morning on
an empty stomach, to mimic the natural fasting-induced AMPK activation.
Product Description – O-304
Synonyms: 4-chloro-N-(2-(4-chlorobenzyl)-3-oxo-2,3-dihydro-1,2,4-thiadiazol-5-yl)benzamide
Molecular Formula: C16H11Cl2N3O2S
Molecular Weight: 380.2 g/mol
CAS Number: 1261289-04-6
PubChem ID: 50923806
Total Active Ingredient: 4500 mg per container (150 mg per capsule)
Shelf Life: 36 months
O-304 Structures
Source: PubChem
Product Usage
This item is supplied for research purposes only.
Peptide Storage
All information provided by PRG is for educational and informational purposes only.
Best Practices for Storing Peptides
To maintain the reliability of laboratory results, correct peptide storage is essential. Proper storage conditions help preserve peptide stability for years while protecting against contamination, oxidation, and breakdown. Although certain peptides are more sensitive than others, following these best practices will greatly extend their shelf life and structural integrity.
- Short-Term Storage (days to months): Keep peptides cool and protected from light. Temperatures below 4 °C (39 °F) are generally suitable. Lyophilized peptides often remain stable at room temperature for several weeks, but refrigeration is still preferred if use is not immediate.
- Long-Term Storage (months to years): Store peptides at –80 °C (–112 °F) for maximum stability. Avoid frost-free freezers, as defrost cycles can cause damaging temperature fluctuations.
- Minimize Freeze–Thaw Cycles: Repeated freezing and thawing accelerates degradation. Instead, divide peptides into aliquots before freezing.
Preventing Oxidation & Moisture Damage
Peptides can be compromised by exposure to moisture and air—especially immediately after removal from a freezer.
- Let the vial warm to room temperature before opening to prevent condensation.
- Keep containers sealed as much as possible, and if possible, reseal under a dry, inert gas such as nitrogen or argon.
- Amino acids like cysteine (C), methionine (M), and tryptophan (W) are particularly sensitive to oxidation.
Storing Peptides in Solution
Peptides in solution have a much shorter lifespan compared to lyophilized form and are prone to bacterial degradation.
- If storage in solution is unavoidable, use sterile buffers at pH 5–6.
- Prepare single-use aliquots to avoid repeated freeze–thaw cycles.
- Most peptide solutions are stable for up to 30 days at 4 °C (39 °F), but sensitive sequences should remain frozen when not in use.
Containers for Peptide Storage
Select containers that are clean, intact, chemically resistant, and appropriately sized for the sample.
- Glass vials: offer clarity, durability, and chemical resistance.
- Plastic vials: polystyrene (clear but less resistant) or polypropylene (translucent but chemically resistant).
- Peptides shipped in plastic vials may be transferred to glass for long-term storage if desired.
Regenesis Peptide Storage Quick Tips
- Keep peptides in a cold, dry, dark environment
- Avoid repeated freeze–thaw cycles
- Minimize exposure to air
- Protect from light
- Avoid storing in solution long term
- Aliquot peptides to match experimental needs