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Phosphate Buffered Saline (PBS) - 20 ml
€15,00
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Overview: Phosphate Buffered Saline (PBS)
Phosphate Buffered Saline is one of the most commonly used solutions for peptide or small molecule reconstitution in laboratory research. By maintaining a constant pH and osmolarity, PBS provides a controlled environment that preserves the structure and function of biological samples.
In cell culture research, PBS is essential for washing and resuspending cells without causing osmotic stress. In protein and molecular biology studies, it serves as a reliable diluent that minimizes interference in downstream assays. Because of its compatibility with enzymes, antibodies, and nucleic acids, PBS is a standard reagent in fields ranging from immunology and biochemistry to microscopy and diagnostics.
Research has consistently demonstrated that PBS’s isotonic properties support reproducibility and reduce variability across experiments, making it an indispensable tool in modern laboratories.
See other buffered saline solution: Histidine Buffered Saline (HBS) - 20 ml
Product Usage
This item is supplied for research purposes only.
Peptide Storage
All information provided by PRG is for educational and informational purposes only.
Best Practices for Storing Peptides
To maintain the reliability of laboratory results, correct peptide storage is essential. Proper storage conditions help preserve peptide stability for years while protecting against contamination, oxidation, and breakdown. Although certain peptides are more sensitive than others, following these best practices will greatly extend their shelf life and structural integrity.
- Short-Term Storage (days to months): Keep peptides cool and protected from light. Temperatures below 4 °C (39 °F) are generally suitable. Lyophilized peptides often remain stable at room temperature for several weeks, but refrigeration is still preferred if use is not immediate.
- Long-Term Storage (months to years): Store peptides at –80 °C (–112 °F) for maximum stability. Avoid frost-free freezers, as defrost cycles can cause damaging temperature fluctuations.
- Minimize Freeze–Thaw Cycles: Repeated freezing and thawing accelerates degradation. Instead, divide peptides into aliquots before freezing.
Preventing Oxidation & Moisture Damage
Peptides can be compromised by exposure to moisture and air—especially immediately after removal from a freezer.
- Let the vial warm to room temperature before opening to prevent condensation.
- Keep containers sealed as much as possible, and if possible, reseal under a dry, inert gas such as nitrogen or argon.
- Amino acids like cysteine (C), methionine (M), and tryptophan (W) are particularly sensitive to oxidation.
Storing Peptides in Solution
Peptides in solution have a much shorter lifespan compared to lyophilized form and are prone to bacterial degradation.
- If storage in solution is unavoidable, use sterile buffers at pH 5–6.
- Prepare single-use aliquots to avoid repeated freeze–thaw cycles.
- Most peptide solutions are stable for up to 30 days at 4 °C (39 °F), but sensitive sequences should remain frozen when not in use.
Containers for Peptide Storage
Select containers that are clean, intact, chemically resistant, and appropriately sized for the sample.
- Glass vials: offer clarity, durability, and chemical resistance.
- Plastic vials: polystyrene (clear but less resistant) or polypropylene (translucent but chemically resistant).
- Peptides shipped in plastic vials may be transferred to glass for long-term storage if desired.
Regenesis Peptide Storage Quick Tips
- Keep peptides in a cold, dry, dark environment
- Avoid repeated freeze–thaw cycles
- Minimize exposure to air
- Protect from light
- Avoid storing in solution long term
- Aliquot peptides to match experimental needs