Histidine Buffered Saline (HBS) - 20 ml
Overview: Histidine Buffered Saline (HBS)
Histidine Buffered Saline has become increasingly important in pharmaceutical and protein research because of its buffering capacity in the slightly acidic to neutral range. Histidine’s imidazole side chain allows precise pH regulation, making HBS a preferred choice in studies where protein stability and biological activity must be preserved.
In peptide and antibody research, HBS helps maintain molecular integrity by reducing denaturation and aggregation, which can occur in less stable buffers. It has also been applied in formulation studies, where maintaining consistent conditions is crucial for reproducibility and accurate data collection.
Comparative research has shown that histidine buffers may outperform phosphate buffers in scenarios involving sensitive biomolecules, particularly when phosphate interference could alter experimental outcomes. For this reason, HBS is widely used in biotechnology, immunology, and biochemical laboratories as a reliable, research-grade buffer solution.
See other bufferes saline solution products: Phosphate Buffered Saline (PBS) - 20 ml
Product Usage
This item is supplied for research purposes only and is intended solely for in vitro studies and laboratory experiments. All information provided on this website is for educational use only. Any form of administration to humans or animals is strictly prohibited by law. This product must only be handled by licensed, qualified professionals. It is not a drug, food, or cosmetic, and must not be misrepresented, mislabeled, or used as such.
Peptide Storage
All information provided by Regenesis is for educational and informational purposes only. All Regenesis products are supplied for in-vitro research use only—meaning experiments conducted outside of a living organism (“in glass”). These compounds are not medicines or drugs, have not been evaluated by the FDA, and are not intended to diagnose, treat, cure, or prevent any disease. Any administration to humans or animals is strictly prohibited by law.
Best Practices for Storing Peptides
To maintain the reliability of laboratory results, correct peptide storage is essential. Proper storage conditions help preserve peptide stability for years while protecting against contamination, oxidation, and breakdown. Although certain peptides are more sensitive than others, following these best practices will greatly extend their shelf life and structural integrity.
- Short-Term Storage (days to months): Keep peptides cool and protected from light. Temperatures below 4 °C (39 °F) are generally suitable. Lyophilized peptides often remain stable at room temperature for several weeks, but refrigeration is still preferred if use is not immediate.
- Long-Term Storage (months to years): Store peptides at –80 °C (–112 °F) for maximum stability. Avoid frost-free freezers, as defrost cycles can cause damaging temperature fluctuations.
- Minimize Freeze–Thaw Cycles: Repeated freezing and thawing accelerates degradation. Instead, divide peptides into aliquots before freezing.
Preventing Oxidation & Moisture Damage
Peptides can be compromised by exposure to moisture and air—especially immediately after removal from a freezer.
- Let the vial warm to room temperature before opening to prevent condensation.
- Keep containers sealed as much as possible, and if possible, reseal under a dry, inert gas such as nitrogen or argon.
- Amino acids like cysteine (C), methionine (M), and tryptophan (W) are particularly sensitive to oxidation.
Storing Peptides in Solution
Peptides in solution have a much shorter lifespan compared to lyophilized form and are prone to bacterial degradation.
- If storage in solution is unavoidable, use sterile buffers at pH 5–6.
- Prepare single-use aliquots to avoid repeated freeze–thaw cycles.
- Most peptide solutions are stable for up to 30 days at 4 °C (39 °F), but sensitive sequences should remain frozen when not in use.
Containers for Peptide Storage
Select containers that are clean, intact, chemically resistant, and appropriately sized for the sample.
- Glass vials: offer clarity, durability, and chemical resistance.
- Plastic vials: polystyrene (clear but less resistant) or polypropylene (translucent but chemically resistant).
- Peptides shipped in plastic vials may be transferred to glass for long-term storage if desired.
Regenesis Peptide Storage Quick Tips
- Keep peptides in a cold, dry, dark environment
- Avoid repeated freeze–thaw cycles
- Minimize exposure to air
- Protect from light
- Avoid storing in solution long term
- Aliquot peptides to match experimental needs